Larkin was born in London in the early 1580s, and lived in the parishes of St Sepulchre-without-Newgate, Holborn, and St Anne Blackfriars. He became a freeman of the Worshipful Company of Painter-Stainers on 7 July 1606 under the patronage of Lady Arbella Stuart and Edward Seymour, 1st Earl of Hertford. Married before 1612, he buried a stillborn son in that year; a son, William, in 1613; and a daughter, Mary, in January 1614/15, all at St Anne Blackfriars. Another daughter called Mary was alive at the time of his death. He died sometime between the witnessing of his will on 10 April 1619 and its proving on 14 May. The date of his burial is unknown because the parish records were destroyed in the Great Fire of London in 1666.