New York City-based punk rock band Ramones used "The Ecstasy of Gold" as the outro music for their concerts since 1991. This can be heard at the end of their live albums Loco Live, Greatest Hits Live and We're Outta Here!. The band uses "The Good, the Bad and the Ugly", another one of Morricone's compositions at the beginning of their concerts since the same year.
Famous cellist Yo-Yo Ma recorded this song in his tribute album, Yo-Yo Ma Plays Ennio Morricone.
The episode "XCIII" in Samurai Jack's5th season pays homage to the cemetery scene when Jack hides from the Daughters of Aku in a stone coffin within a ruined temple (complete with a reimagining of The Ecstasy of Gold).