A spore print is made by placing the spore-producing surface flat on a sheet of dark and white paper or on a sheet of clear, stiff plastic, which facilitates moving the spore print to a darker or lighter surface for improved contrast; for example, it is easier to determine whether the spore print is pure white or, rather, very slightly pigmented. The mushroom is left for several hours, often overnight, in this manner. Some guides advise using a moisture-resistant enclosure, like a glass or jar, to contain the mushroom during printing. If the mushroom is to be preserved, a small hole can be made in the spore print paper rather than cutting the stipe. When the mushroom is removed, the color of the spores should be visible. Mycologists often use glass slides, which allow for quick examination of spores under a microscope. A mushroom cannot be identified from its spore print alone; the spore print is only one characteristic used in making a taxonomic determination. Spore prints are usually white to cream, black, or shaded of red, purple, or brown. The poisonous false parasol (Chlorophyllum molybdites) has a green spore deposit. Although the spore print is generally used to help identify the genus of a specimen, on occasion it can be used to help distinguish between similar species. For example, the lookalikes Russula crustosa and Russula virescens have yellowish and white spore deposits, respectively.
In the field, spore print color can sometimes be quickly determined by examining the top of stipes for spore "dust" on the nearby forest floor.
The spore print is not always successful, as some mushrooms are too young or old to produce spores. Mushrooms collected at high elevations will typically not produce a spore print at lower elevations. Sometimes, the color can vary depending on the thickness of the spore print. In the genus Lactarius, thin deposits are usually white, while thick deposits are cream to yellow. Many of the original authors of species in this genus did not indicate whether spore print colors they recorded were with thick or thin prints, limiting the usefulness of this feature as an identification characteristic. In some instances, the color of the spore print matches that of the mature gills, but this is not always true and gill color cannot reliably be used as an indicator of spore print color.