adipose tissue, intracellular triglycerides are stored in cytoplasmic
lipid droplets. When
lipases are phosphorylated, they access lipid droplets and through multiple steps of hydrolysis, breakdown triglycerides into fatty acids and glycerol. Each step of hydrolysis leads to the removal of one fatty acid. The first step and the rate-limiting step of lipolysis is carried out by
adipose triglyceride lipase (ATGL). This enzyme catalyzes the hydrolysis of
hormone-sensitive lipase (HSL) catalyzes the hydrolysis of diacylglycerol to
monoacylglycerol lipase (MGL) catalyzes the hydrolysis of monoacylglycerol to
 Perilipin 1A is a key protein regulator of lipolysis in adipose tissue. This lipid droplet-associated protein, when deactivated, will prevent the interaction of lipases with triglycerides in the lipid droplet and grasp the ATGL co-activator, comparative gene identification 58 (CGI-58) (a.k.a.
ABHD5). When perilipin 1A is phosphorylated by PKA, it releases CGI-58 and it expedites the docking of phosphorylated lipases to the lipid droplet.
 CGI-58 can be further phosphorylated by PKA to assist in its dispersal to the cytoplasm. In the cytoplasm, CGI-58 can co-activate ATGL.
 ATGL activity is also impacted by the negative regulator of lipolysis, G0/G1 switch gene 2 (G0S2). When expressed, G0S2 acts as a competitive inhibitor in the binding of CGI-58.
 Fat-specific protein 27 (FSP-27) (a.k.a CIDEC) is also a negative regulator of lipolysis. FSP-27 expression is negatively correlated with ATGL mRNA levels.